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1 article(s) from Hassenrück, Jessica

Cyclopropene derivatives of aminosugars for metabolic glycoengineering

Jessica Hassenrück and
Valentin Wittmann

Beilstein J. Org. Chem. 2019, 15, 584–601, doi:10.3762/bjoc.15.54

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Graphical Abstract

Figure 1: Cyclopropene-modified mannosamine, glucosamine and galactosamine derivatives employed for MGE.

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Figure 2: A) Reaction of ManNCyc and ManNCp, respectively, with Tz-PEG-OH to determine second-order rate cons...

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Scheme 1: MGE with cyclopropene-modified mannosamines. Cells were grown with sugar for 48 hours and then incu...

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Figure 3: HEK 293T cells were grown with 100 μM Ac₄ManNCyc, Ac₄ManNCp, Ac₄ManNCyoc or DMSO only (negative con...

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Scheme 2: Synthesis of Ac₄ManNCp(H₂) and Ac₄ManNCyc(H₂) and the corresponding DMB-labeled sialic acids. C/A =...

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Scheme 3: Synthesis of Ac₄ManNCyoc(H₂) and the corresponding DMB-labeled sialic acid.

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Scheme 4: Synthesis of Ac₄GlcNCp and Ac₄GalNCp.

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Figure 4: HEK 293T cells were grown with 100 μM Ac₄ManNCp, Ac₄GlcNCp, Ac₄GalNCp or DMSO only (negative contro...

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Figure 5: HEK 293T cells were grown with 100 μM Ac₄GlcNCp, Ac₄GalNCp or DMSO only (negative control) for 48 h...

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Figure 6: HEK 293T cells were grown with 50 μM (A) or 100 μM (B) Ac₄GlcNCp, Ac₄GlcNCyoc or DMSO only (negativ...

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Figure 7: Western blot analysis of soluble glycoproteins. HEK 293T cells were grown for 48 h with 100 μM Ac₄M...

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Scheme 5: Synthesis of Ac₄GlcNCp(H₂) and Ac₄GlcNCyoc(H₂).

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Published 04 Mar 2019

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